Our results suggest that AMI likely stimulated bone marrow, and MNC CD34+ were subsequently mobilized.Ĭurrently, little is known about how MNC CD34+ are mobilized after AMI. Circulating MNC CD34+ counts significantly increased in patients with AMI, peaking on day 7, but they did not change in controls. Plasma levels of vascular endothelial growth factor significantly increased, peaking on day 7, and they positively correlated with circulating MNC CD34+ counts ( r=0.35, P=0.01).Ĭonclusions-This is the first clinical demonstration showing that lineage-committed EPCs and MNC CD34+, their putative precursors, are mobilized during an acute ischemic event in humans.īecause EPCs differentiate from MNC CD34+ and mobilize from bone marrow, 2 3 we first quantified circulating MNC CD34+. In patients with acute myocardial infarction, more cell clusters and EPCs developed from cultured PB-MNCs obtained on day 7 than those on day 1. During culture, PB-MNCs formed multiple cell clusters, and EPC-like attaching cells with endothelial cell lineage markers (CD31, vascular endothelial cadherin, and kinase insert domain receptor) sprouted from clusters. Methods and Results-Flow cytometry revealed that circulating MNC CD34+ counts significantly increased in patients with acute myocardial infarction (n=16), peaking on day 7 after onset, whereas they were unchanged in control subjects (n=8) who had no evidence of cardiac ischemia. However, little is known regarding whether EPCs and their putative precursor, CD34-positive mononuclear cells (MNC CD34+), are mobilized into PB in acute ischemic events in humans. Customer Service and Ordering Informationīackground-Endothelial progenitor cells (EPCs) circulate in adult peripheral blood (PB) and contribute to neovascularization.Stroke: Vascular and Interventional Neurology.Journal of the American Heart Association (JAHA).Circ: Cardiovascular Quality & Outcomes.Arteriosclerosis, Thrombosis, and Vascular Biology (ATVB).
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